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Effects of PCB 52 and PCB 77 on Cell Viability, [Ca(2+)](i) Levels and Membrane Fluidity in Mouse Thymocytes

Effects of PCB 52 and PCB 77 on Cell Viability, [Ca(2+)](i) Levels and Membrane Fluidity in Mouse Thymocytes

Bayram Yilmaz, Suleyman Sandal, Chang-Hwei Chen, David O Carpenter

Toxicology. 2006 Jan 16;217(2-3):184-93.

PMID: 16233944

Abstract:

Exposure to polychlorinated biphenyls (PCBs) is known to suppress immune system function and this action is usually ascribed to dioxin-like PCBs that act via the Ah receptor. We have studied the effects of one ortho-substituted, non-dioxin-like PCB (PCB 52) and one coplanar, dioxin-like congener (PCB 77) on properties of thymocytes acutely isolated from mice. Viability of thymocytes was dose- and time-dependently reduced by PCB 52 with a threshold concentration of about 1 microM, while there was no effect of PCB 77 on viability at concentrations less than 10 microM. Cell death was detectible within 5 min of exposure. Both congeners caused a dose-dependent increase in [Ca(2+)](i), but the threshold concentration was 1 microM for PCB 52 and 5 microM for PCB 77. However, the cell death was not due to the elevation of [Ca(2+)](i), since it was not reduced by incubation in Ca-free Tyrode's Solution. PCB 52, but not PCB 77, caused an increase in membrane fluidity at a concentration of 5 microM. These observations are consistent with previous results that suggest that ortho-substituted PCB congeners dissolve in cell membrane and cause greater disruption of function than do dioxin-like PCB congeners.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP35693993-B	PCB No 52 solution		35693-99-3	Price

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