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[Effects of PEG and NaCl Stress on the Contents of Indigotin and Indirubin in Isatis Indigotica Tetraploids]

[Effects of PEG and NaCl Stress on the Contents of Indigotin and Indirubin in Isatis Indigotica Tetraploids]

Shao-Ying Ke, Yan-Zhi Ma

Zhong Yao Cai. 2013 Apr;36(4):525-7.

PMID: 24133992

Abstract:

Objective:
To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..
Methods:
The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC.
Results:
The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased.
Conclusion:
DB3 and DB12 can be further studied as reproduction materials.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP482893	Indigotin		482-89-3	Price

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