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Effects of Prunetin on the Proteolytic Activity, Secretion and Gene Expression of MMP-3 in Vitro and Production of MMP-3 in Vivo

Effects of Prunetin on the Proteolytic Activity, Secretion and Gene Expression of MMP-3 in Vitro and Production of MMP-3 in Vivo

Dae Cheol Nam, Bo Kun Kim, Hyun Jae Lee, Hyun-Dae Shin, Choong Jae Lee, Sun-Chul Hwang

Korean J Physiol Pharmacol. 2016 Mar;20(2):221-8.

PMID: 26937219

Abstract:

We investigated whether prunetin affects the proteolytic activity, secretion, and gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective eff ect of prunetin. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular chondrocytes, the effects of prunetin on IL-1β-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The eff ect of prunetin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) prunetin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5; (2) prunetin inhibited the secretion and proteolytic activity of MMP-3; (3) prunetin suppressed the production of MMP-3 protein in vivo. These results suggest that prunetin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP552590	Prunetin		552-59-0	Price

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