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Effects of Qingyi Huaji Decoction on Serum Levels of interleukin-6, interleukin-8 and Tumor Necrosis Factor-Alpha in Nude Mice Bearing Pancreatic Tumors

Effects of Qingyi Huaji Decoction on Serum Levels of interleukin-6, interleukin-8 and Tumor Necrosis Factor-Alpha in Nude Mice Bearing Pancreatic Tumors

Hua-Qiang Ouyang, Lu-Ming Liu, Zhen Chen, Jian-Min Luo, Er-Xin Yu

Zhong Xi Yi Jie He Xue Bao. 2010 Jul;8(7):655-61.

PMID: 20619142

Abstract:

Objective:
To investigate the effects of Qingyi Huaji (QYHJ) decoction, a compound traditional Chinese herbal medicine, on tumor inhibition rate and serum levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) in nude mice with transplanted tumors of human pancreatic cancer.
Methods:
The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of pancreatic cancer into the right armpit of 40 BALB/c nude mice. After successful modeling, the mice were randomly divided into untreated group (Arabic gum), capecitabine group, low-dose QYHJ decoction group (36 g/kg) and high-dose QYHJ decoction group (72 g/kg), with 10 mice in each group. Citrate buffer solution (containing 5% Arabic gum), capecitabine suspension and QYHJ decoction were administered to four groups by gavage respectively. After 5-week treatment, the concentrations of serum IL-6, IL-8 and TNF-alpha were examined by enzyme-linked immunosorbent assay (ELISA) using blood sample from eye socket. Then the mice were euthanized by cervical dislocation. Tumor weight and the tumor inhibition rate were calculated.
Results:
Tumor weight in the low-dose QYHJ decoction group decreased significantly as compared with the untreated group (P<0.05). Serum levels of IL-6 and TNF-alpha in low- and high-dose QYHJ groups were extremely significantly lower than those in the untreated group (P<0.01). Serum level of IL-8 in the low-dose QYHJ group was significantly lower than that in the untreated group (P<0.05). Correlation analysis showed that transplanted tumor weight of the mice was linearly positively correlated with serum levels of IL-6, IL-8 or TNF-alpha (P<0.01).
Conclusion:
Conventional dose of QYHJ decoction is effective in suppressing pancreatic carcinoma in nude mice. The mechanism may be related to down-regulation of serum cytokines such as IL-6, IL-8 and TNF-alpha.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP921769655	Capecitabine Related Compound C		921769-65-5	Price

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