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Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells

Rong Hu, Yan Lou, Fei-Miao Wang, Hui-Ming Ma, Xing Wu, Xiao-Mei Zhang, Juan Li, Ying-Pu Sun

Cell Biochem Biophys. 2013;67(3):1481-5.

PMID: 23723001

Abstract:

The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42413281 SCF human SCF human Price
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