Home
Resources
Publications
[Effects of Sophoricoside and Genistein on Biological Characteristics of Osteoblasts]

[Effects of Sophoricoside and Genistein on Biological Characteristics of Osteoblasts]

Yong Xu, Wei-zhen Chen, Ning Du

Zhong Xi Yi Jie He Xue Bao. 2009 Mar;7(3):223-7.

PMID: 19284950

Abstract:

Objective:
To observe the effects of sophoricoside and genistein on the biological characteristics of osteoblasts.
Methods:
Osteoblasts were isolated from parietal bone of the newborn SD rats (less than 24 hours) and cultured in minimum essential medium (MEM). The effects of different concentrations of sophoricoside and genistein (10, 1 and 0.1 micromol/L) on the bone formation of osteoblasts in vitro were observed by cell proliferation rate. Differentiation of osteoblasts was evaluated by detecting the activity of alkaline phosphatase (ALP) using 4-nitrophenyl phosphate disodium salt (PNPP) method and by measuring the mineralized nodiform dyed by alizarin bordeaux. And the activity of osteoprotegerin (OPG) promoters of human beings was tested by using gene cloning and reporter gene.
Results:
Sophoricoside at 1 and 0.1 micromol/L could promote the proliferation of osteoblasts (vs control group, P < 0.05). Genistein at 10 micromol/L could inhibit the cell proliferation, while genistein at 0.1 micromol/L showed promotion to cell proliferation (vs control group, P < 0.05). The activity of ALP of osteoblasts was increased by sophoricoside at concentrations from 0.1 micromol/L to 10 micromol/L, but genistein only at concentration of 0.1 micromol/L could make it. The total areas of mineralization of the osteoblasts in 10, 1 and 0.1 micromol/L sophoricoside groups increased by 73%, 138.6% and 114.3% as compared with the control group, respectively, but those in different concentrations of genistein groups decreased. The activity of LacZ was increased by sophoricoside at concentrations from 0.1 micromol/L to 10 micromol/L, as well as by genistein at concentration of 0.1 micromol/L.
Conclusion:
Sophoricoside can stimulate the proliferation, differentiation and mineralization of osteoblasts. And it shows no inhibition to bone formation of osteoblasts compared with genistein. Sophoricoside can also increase the activity of osteoprotegerin promoter and is more effective than genistein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP152954	Sophoricoside		152-95-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: