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Effects of the Local Anesthetic Tetracaine on the Structural and Dynamic Properties of Lipids in Model Membranes: A High-Pressure Fourier Transform Infrared Study

Effects of the Local Anesthetic Tetracaine on the Structural and Dynamic Properties of Lipids in Model Membranes: A High-Pressure Fourier Transform Infrared Study

M Auger, H C Jarrell, I C Smith, D J Siminovitch, H H Mantsch, P T Wong

Biochemistry. 1988 Aug 9;27(16):6086-93.

PMID: 3191108

Abstract:

High-pressure Fourier transform infrared (FT-IR) spectroscopy was used to study the effects of a local anesthetic, tetracaine, on the structural and dynamic properties of lipids in model membranes. The model membrane systems studied were multilamellar aqueous dispersions of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine (DHPC) in the absence and presence of a physiological concentration of cholesterol (30 mol %). The infrared spectra were measured at 28 degrees C in a diamond anvil cell as a function of pressure up to 25 kbar. The results indicate that the effects of tetracaine on the structure of pure DMPC bilayers in the gel state are dependent on the state of charge of the anesthetic. The uncharged tetracaine disorders the lipid acyl chains while the charged form induces the formation of an interdigitated gel phase. The presence of cholesterol in the latter system prevents the formation of the interdigitated phase, whereas in the former system it disorders the lipid acyl chains in the gel state. Moreover, it is shown that the addition of uncharged tetracaine to interdigitated DHPC bilayers does not alter the interdigitated state of the hydrocarbon chains.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP36314473	1,2-Di-O-hexadecyl-sn-glycero-3-phosphocholine		36314-47-3	Price

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