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Efficient Biosynthesis of 3, 4-dihydroxyphenylacetic Acid in Escherichia Coli

Efficient Biosynthesis of 3, 4-dihydroxyphenylacetic Acid in Escherichia Coli

Xianglai Li, Xiaolin Shen, Jia Wang, Hyon Il Ri, Chang Yong Mi, Yajun Yan, Xinxiao Sun, Qipeng Yuan

J Biotechnol. 2019 Mar 20;294:14-18.

PMID: 30771441

Abstract:

3, 4-Dihydroxyphenylacetic acid (3, 4-DHPA) is a phenolic acid with strong anti-oxidative activity, showing potential applications in food and pharmaceutical industries. In this study, a 3, 4-DHPA biosynthetic pathway was designed by connecting 4-hydroxyphenylacetic acid (4-HPA) biosynthesis with its hydroxylation. The starting strain produced only 46 mg/L of 4-HPA in 48 h. Enhancing the shikimate pathway increased the titer by 19-fold to 923 ± 57 mg/L. Furthermore, pykA and pykF were disrupted to conserve phosphoenolpyruvate for 4-HPA production. With this effort, 4-HPA titer was increased to 1817 ± 55 mg/L. Introducing the hydroxylase HpaBC into the 4-HPA overproducing strain resulted in 3, 4-DHPA production and the best strain produced 1856 ± 67 mg/L of 3, 4-DHPA in shake flask cultures. This work reports de novo biosynthesis of 3, 4-DHPA for the first time and provides a promising alternative for sustainable production of this valuable compound.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP182163968	3,4,5-Trimethoxyphenylboronic acid		182163-96-8	Price

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