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Efficient Preparation of Rare Sagittatoside A From Epimedin A, by Recyclable Aqueous Organic Two-Phase Enzymatic Hydrolysis

Efficient Preparation of Rare Sagittatoside A From Epimedin A, by Recyclable Aqueous Organic Two-Phase Enzymatic Hydrolysis

Yuping Shen, Yi Lu, Jing Gao, Yeting Zhu, Man Wang, Shunli Jing, Lili Xu, Huan Yang, Xiaobin Jia

Nat Prod Res. 2019 Nov;33(21):3095-3102.

PMID: 30470129

Abstract:

The rare secondary flavonol glycoside Sagittatoside A has much better in vivo bioactivities than epimedin A in Epimedii Folium. However, its current preparation methods are of low efficiency, with byproducts generated. The aim of this study was to establish a novel catalysis system for effective and convenient preparation of Sagittatoside A from epimedin A based on recyclable and integrated aqueous organic two-phase enzymatic hydrolysis. The system was consisted of propyl acetate and HAc-NaAc buffer (pH4.5) containing β-dextranase/epimedin A, and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin A was completely hydrolyzed to sagittatoside A, and 95.02% of the product was transferred into the organic phase. Moreover, 90% of its initial activity was retained after seven cycles of hydrolysis. Additionally, the procedure was simpler than conventional enzymatic hydrolysis. Collectively, the newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside A in industrial application.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP110623728	Epimedin A		110623-72-8	Price

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