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Efficient Production of Fatty Acid Methyl Esters by a Wastewater-Isolated Microalgae-Yeast Co-Culture

Jessica K Suastes-Rivas, Raúl Hernández-Altamirano, Violeta Y Mena-Cervantes, Ruby Valdez-Ojeda, Tanit Toledano-Thompson, Luis R Tovar-Gálvez, Silvia López-Adrián, Isaac Chairez

Environ Sci Pollut Res Int. 2019 Dec 16.

PMID: 31845266

Abstract:

Improving the competitiveness of biodiesel production by microalgae cultures requires the application of several strategies to obtain a high content of lipids, rapid biomass growth and a capacity to adapt to different kinds of environment, with the aim of using non-renewable nutrient sources. Therefore, the use of an individual indigenous microalgae strain or a consortium from natural or anthropogenic sites is now considered an alternative for biofuel production. This study examined the temporal behaviour of secondary metabolites produced by a native microalgae and yeast consortium isolated from wastewater, which was characterized by a genetic identification method based on the MiSeq system. The predominant species in the consortium was Scenedesmus obliquus, representing 68% of the organisms. In addition, the consortium contained a number of yeast species, including Candida pimensis (43%), Arthroderma vanbreuseghemii (23%), Diaporthe aspalathi/Diaporthe meridionalis (25%) and Hericium americanum (3%). This indigenous co-culture of microalgae and yeast showed biomass productivity of 0.06 g l-1 day-1, with a content of 30% (w/w) carbohydrates, 4% (w/w) proteins and 55% (w/w) lipids. Transesterification of the extracted lipids produced fatty acid methyl esters (FAMEs), which were analysed by gas chromatography (GC). The FAMEs included methyl pentadecanoate (1.90%), cis-10-pentanedecanoic acid methyl ester (1.36%), methyl palmitate (2.64%), methyl palmitoleate (21.36%), methyl oleate (64.95%), methyl linolenate (3.83%) and methyl linolelaidate (3.95%). This composition was relevant for biodiesel production based on the co-culture of indigenous microalgae and yeast consortia.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP1120258-A Methyl palmitoleate Methyl palmitoleate 1120-25-8 Price
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