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Efficient Production of Recombinant Human Transcription Factor IIE

Woo J Moon, Jessica A Apostol, Aaron J McBride, Lata I Shukla, Arik Dvir, Zachary F Burton

Protein Expr Purif. 2004 Apr;34(2):317-23.

PMID: 15003267

Abstract:

Transcription factor IIE (TFIIE) is a general initiation and promoter escape factor for RNA polymerase II composed of p56 (TFIIE-alpha) and p34 (TFIIE-beta) subunits. Our laboratories experienced difficulty producing adequate quantities of recombinant human TFIIE-alpha for in vitro studies using available clones. We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression. We report a much-improved system for production of pure, soluble, and active TFIIE complex for in vitro studies.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42414916 TFIIE β, p34 human TFIIE β, p34 human Price
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