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Efficient Purification of Lysozyme From Egg White by 2-mercapto-5-benzimidazolesulfonic Acid Modified Fe3O4/Au Nanoparticles

Efficient Purification of Lysozyme From Egg White by 2-mercapto-5-benzimidazolesulfonic Acid Modified Fe3O4/Au Nanoparticles

Xinjun Zhu, Lianying Zhang, Aiyun Fu, Hao Yuan

Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:213-217.

PMID: 26652366

Abstract:

2-Mercapto-5-benzimidazolesulfonic acid (MBISA) modified Fe3O4/Au nanoparticles were synthesized in aqueous solution and characterized by photo correlation spectroscopy (PCS) and vibrating sample magnetometer (VSM). The so-obtained Fe3O4/Au-MBISA nanoparticles were capable of specific adsorbing lysozyme. The maximum amount of lysozyme adsorbed on 1.0mg Fe3O4/Au-MBISA nanoparticles was 346μg. The lysozyme desorption behavior was studied and the lysozyme recovery from Fe3O4/Au-MBISA nanoparticles approached 100% under optimal conditions, and the reusability studies showed that the nanoparticles could maintain about 91% of the initial lysozyme adsorption capacity after 7 repeated adsorption-elution cycles. The Fe3O4/Au-MBISA nanoparticles were used in the purification of lysozyme from chicken egg white, which was verified by a single SDS-PAGE band. Therefore, the obtained Fe3O4/Au-MBISA nanoparticles exhibited excellent performance in the direct purification of lysozyme from egg white.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP12650883-A	Lysozyme from chicken egg white		12650-88-3	Price

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