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Electro-activation of Potassium Acetate, Potassium Citrate and Calcium Lactate: Impact on Solution Acidity, Redox Potential, Vibrational Properties of Raman Spectra and Antibacterial Activity on

Electro-activation of Potassium Acetate, Potassium Citrate and Calcium Lactate: Impact on Solution Acidity, Redox Potential, Vibrational Properties of Raman Spectra and Antibacterial Activity on

Viacheslav Liato, Steve Labrie, Mohammed Aïder

Springerplus. 2016 Oct 10;5(1):1760.

PMID: 27833832

Abstract:

Aims:
To study the electro-activation of potassium acetate, potassium citrate and calcium lactate aqueous solutions and to evaluate their antimicrobial effect against E. coli O157:H7 at ambient temperature.
Methods and results:
Potassium acetate, potassium citrate and calcium lactate aqueous solutions were electrically excited in the anodic compartment of a four sectional electro-activation reactor. Different properties of the electro-activated solutions were measured such as: solutions acidity (pH and titratable), Redox potential and vibrational properties by Raman spectroscopy. Moreover, the antimicrobial activity of these solutions was evaluated against E. coli O157:H7. The results showed a pH decrease from 7.07 ± 0.08, 7.53 ± 0.12 and 6.18 ± 0.1 down to 2.82 ± 0.1, 2.13 ± 0.09 and 2.26 ± 0.15, after 180 min of electro-activation of potassium acetate, potassium citrate and calcium lactate solution, respectively. These solutions were characterized by high oxidative ORP of +1076 ± 12, +958 ± 11 and +820 ± 14 mV, respectively. Raman scattering analysis of anolytes showed stretching vibrations of the hydrogen bonds with the major changes within the region of 3410-3430 cm-1. These solutions were used against E. coli O157:H7 and the results from antimicrobial assays showed high antibacterial effect with a population reduction of ≥6 log CFU/ml within 5 min of treatment.
Conclusions:
This study demonstrated the effectiveness of the electro-activation to confer to aqueous solutions of organic salts of highly reactive properties that differ them from their conjugated commercial acids. The electro-activated solutions demonstrated significant antimicrobial activity against E. coli O157:H7.
Significance and impact of study:
This study opens new possibilities to use electro-activated solutions of salts of weak organic acids as food preservatives to develop safe, nutritive and low heat processed foods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP127082-B	Potassium acetate		127-08-2	Price

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