Home
Resources
Publications
Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine

Electrochemical Quantification of Glycated and Non-glycated Human Serum Albumin in Synthetic Urine

Aisha M Attar, Mark B Richardson, Gaetano Speciale, Sudipta Majumdar, Rebekah P Dyer, Emily C Sanders, Reginald M Penner, Gregory A Weiss

ACS Appl Mater Interfaces. 2019 Feb 6;11(5):4757-4765.

PMID: 30668098

Abstract:

A polymer-based electrode capable of specific detection of human serum albumin, and its glycated derivatives, is described. The sensor is constructed from a glass microscope slide coated with a synthesized, polythiophene film bearing a protected, iminodiacetic acid motif. The electrode surface is then further elaborated to a functional biosensor through deprotection of the iminodiacetic acid, followed by metal-affinity immobilization of a specific and high-affinity, albumin ligand. Albumin was then quantified in buffer and synthetic urine via electrochemical impedance spectroscopy. Glycated albumin was next bound to a boronic acid-modified, single-cysteine dihydrofolate reductase variant to quantify glycation ratios by square-wave voltammetry. The platform offers high sensitivity, specificity, and reproducibility in an inexpensive arrangement. The detection limits exceed the requirements for intermediate-term glycemic control monitoring in diabetes patients at 5 and 1 nM for albumin and its glycated forms, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42410748	Albumin, glycated human			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: