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Electron Microscopic Immunoperoxidase Staining of Insulin Using 4-chloro-1-naphthol After Osmium Fixation

Electron Microscopic Immunoperoxidase Staining of Insulin Using 4-chloro-1-naphthol After Osmium Fixation

D G Baskin, H Mar, K C Gorray, W Y Fujimoto

J Histochem Cytochem. 1982 Jul;30(7):710-2.

PMID: 6179987

Abstract:

Ultrastructural localization of insulin in B cells of guinea pig pancreas was accomplished after osmium fixation with an immunoperoxidase procedure that utilized 4-chloro-1-naphthol (CN) in the substrate solution. The principal features of this protocol were: a) osmium tetroxide postfixation; b) omission of hydrogen peroxide "etching"; c) use of CN instead of diaminobenzidine in the substrate solution; d) elimination of osmium tetroxide after the substrate reaction; e) uranyl acetate and lead citrate counterstaining. This procedure produces intense specific staining with low background using highly dilute antiserum, and appears to be useful for postembedding immunoperoxidase staining of a variety of peptide antigens in osmium-fixed tissue.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411223	4-Chloro-1-naphthol solution			Price

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