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Elevated Acetoacetate and Monocyte Chemotactic protein-1 Levels in Cord Blood of Infants of Diabetic Mothers

Elevated Acetoacetate and Monocyte Chemotactic protein-1 Levels in Cord Blood of Infants of Diabetic Mothers

Dalibor Kurepa, Arun K Pramanik, Venkatakrishna Kakkilaya, Gloria Caldito, Lynn J Groome, Joseph A Bocchini, Sushil K Jain

Neonatology. 2012;102(3):163-8.

PMID: 22776897

Abstract:

Background:
Infants of diabetic mothers (IDMs) are at increased risk for metabolic complications. Type 1 and some type 2 diabetic patients have elevated levels of the ketone bodies acetoacetate (AA) and β-hydroxybutyrate (BHB).
Objective:
The aim of this study was to examine how hyperketonemia in diabetic mothers affects markers of inflammation and oxidative stress in their offspring.
Methods:
Blood was obtained from 23 diabetic mothers and 13 healthy mothers and their infants' umbilical cords at delivery. Interleukin-8, monocyte chemotactic protein-1 (MCP-1) and protein carbonyl (protein oxidation) levels were determined by ELISA. U937 human monocyte cell culture was used to examine the effect of AA and BHB on secretion of MCP-1.
Results:
There was a significant increase in the levels of AA in cord blood of IDMs compared with cord blood of infants of healthy mothers. A significant increase in the levels of protein oxidation (p < 0.05) and MCP-1 levels (p < 0.05) was observed in the cord blood of IDMs. The level of MCP-1 correlated significantly (r = 0.51, p = 0.01) with the concentration of AA in the IDMs. In further experiments with cultured monocytes treated with exogenous AA (0-4 mM), a significant increase in MCP-1 secretion was observed in AA- but not BHB-treated monocytes.
Conclusion:
Blood levels of AA and MCP-1 are elevated in IDMs, which may contribute to the development of the metabolic complications seen in IDMs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413133	Monocyte Chemotactic Protein-1 human			Price

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