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Enantiomeric Purity Determination of acetyl-L-carnitine by Reversed-Phase High-Performance Liquid Chromatography Using Chiral Derivatization

Enantiomeric Purity Determination of acetyl-L-carnitine by Reversed-Phase High-Performance Liquid Chromatography Using Chiral Derivatization

M Kagawa, Y Machida, H Nishi

J Chromatogr A. 1999 Oct 1;857(1-2):127-35.

PMID: 10536831

Abstract:

An indirect HPLC enantioseparation method for the determination of acetyl-D-carnitine (D-AC) in acetyl-L-carnitine (L-AC) was developed. L-AC was derivatized with a chiral amino compound which has a chromophore for UV detection. Six chiral amino compounds were examined as chiral derivatization reagents. Among them, enantiomers of acetylcarnitine derivatized with L-alanine-beta-naphthylamide (L-Ala-beta-NA) were successfully separated on an ODS column within 10 min with Rs = 1.94 and alpha = 1.10. Quantitation was achieved through UV detection at 254 nm. The derivatization reaction of L-AC with L-Ala-beta-NA was completed in less than 10 min at room temperature (ca. 20 degrees C). Validation data such as linearity, detection limit, and precision are also presented. The detection limit of D-AC in L-AC in this method was below 0.05% (visual evaluation). This method was found to be applicable as a practical quality control method for the enantiomeric purity determination of L-AC.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP720821	L-Alanine β-naphthylamide		720-82-1	Price

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