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Enantioseparation of Omeprazole and Its Metabolite 5-hydroxyomeprazole Using Open Tubular Capillary Electrochromatography With Immobilized Avidin as Chiral Selector

Enantioseparation of Omeprazole and Its Metabolite 5-hydroxyomeprazole Using Open Tubular Capillary Electrochromatography With Immobilized Avidin as Chiral Selector

J Olsson, L G Blomberg

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 1;875(1):329-32.

PMID: 18573699

Abstract:

The present paper demonstrates the enantiomeric separation of omeprazole and its metabolite 5-hydroxyomeprazole performed with open tubular capillary electrochromatography (OT-CEC). The protein avidin was used as the chiral selector. Avidin was immobilized by a Schiffs base type of reaction where the protein was via glutaraldehyde covalently bonded to the amino-modified wall of a fused-silica capillary, 50 microm i.d. Both racemates were baseline resolved. Resolution was 1.9 and 2.3, respectively, using ammonium acetate buffer, pH 5.8, 5% methanol, with UV-detection. These values of resolution using OT-CEC are higher than earlier published results regarding chiral separation of omeprazole and 5-hydroxyomeprazole on packed CEC. The number of theoretical plates also indicated good separation efficiency.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP92340573	5-Hydroxyomeprazole		92340-57-3	Price

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