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Endocytosis Is Not Required for the Selective Lipid Uptake Mediated by Murine SR-BI

Endocytosis Is Not Required for the Selective Lipid Uptake Mediated by Murine SR-BI

Thomas J F Nieland, Marcelo Ehrlich, Monty Krieger, Tomas Kirchhausen

Biochim Biophys Acta. 2005 May 1;1734(1):44-51.

PMID: 15866482

Abstract:

The scavenger receptor class B, type I (SR-BI) mediates the cellular selective uptake of cholesteryl esters and other lipids from high-density lipoproteins (HDL) and low-density lipoproteins (LDL). This process, unlike classical receptor-mediated endocytosis, does not result in lipoprotein degradation. Instead, the lipid depleted particles are released into the medium. Here we show that selective lipid uptake mediated by murine SR-BI can be uncoupled from the endocytosis of HDL or LDL particles. We found that blocking selective lipid uptake by incubating cells with the small chemical inhibitors BLT-1 or BLT-4 did not affect endocytosis of HDL. Similarly, blocking endocytosis by hyperosmotic sucrose or K+ depletion did not prevent selective lipid uptake from HDL or LDL. These findings suggest that mSR-BI-mediated selective uptake occurs at the cell surface upon the association of lipoproteins with mSR-BI and does not require endocytosis of HDL or LDL particles.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP251917790	BLT-4		251917-79-0	Price

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