Home
Resources
Publications
Endothelial Cell Growth Supplement: A Cell Cloning Factor That Promotes the Growth of Monoclonal Antibody Producing Hybridoma Cells

Endothelial Cell Growth Supplement: A Cell Cloning Factor That Promotes the Growth of Monoclonal Antibody Producing Hybridoma Cells

C Pintus, J H Ransom, C H Evans

J Immunol Methods. 1983 Jul 15;61(2):195-200.

PMID: 6863945

Abstract:

The efficiency of endothelial cell growth supplement (ECGS), a commercially marketed extract of bovine neural tissue, human endothelial cell supernatant (HECS) derived from freshly isolated endothelial cells, and feeder layers of murine peritoneal cells (PEC), were compared for their ability to support cell fusion, clonal growth, and monoclonal antibody production of murine hybridoma cells. ECGS at 25-100 micrograms/ml was similar to a 1:5 dilution of HECS in supporting the growth of hybridoma colonies; both ECGS and HECS were superior to PEC feeder cells. Furthermore, hybridomas cloned in ECGS produced anti-lymphotoxin antibodies. The commercial availability and stability of ECGS together with its ability anti-lymphotoxin antibodies. The commercial availability and stability of ECGS a superior growth supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248871	Endothelial cell growth supplement from bovine neural tissue			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: