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Endothelin-3 Is a Novel Neuropeptide: Isolation and Sequence Determination of endothelin-1 and endothelin-3 in Porcine Brain

Endothelin-3 Is a Novel Neuropeptide: Isolation and Sequence Determination of endothelin-1 and endothelin-3 in Porcine Brain

O Shinmi, S Kimura, T Sawamura, Y Sugita, T Yoshizawa, Y Uchiyama, M Yanagisawa, K Goto, T Masaki, I Kanazawa

Biochem Biophys Res Commun. 1989 Oct 16;164(1):587-93.

PMID: 2508640

Abstract:

The molecular forms of endothelin (ET) related peptides were investigated in porcine brain by using high performance liquid chromatography coupled with three specific radioimmunoassays. ET-1 and its oxidized form were isolated and sequenced as in the case of porcine spinal cord. A very small amount of big ET-1 (1-39) and its C-terminal fragment (big ET-1 (22-39] were also detected. Furthermore, immunoreactive (ir)-ET-3 was isolated and sequenced; its partial primary structure was identical to that of human (rat) ET-3. The concentrations of ir-ET-1 and ir-ET-3 in porcine brain were 140 fmol/g tissue and 5 fmol/g tissue, respectively. These results indicate that besides ET-1, ET-3 is a novel neuropeptide in the central nervous system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP117399936-A	Endothelin 3 human, rat		117399-93-6	Price

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