Home
Resources
Publications
Energy-dependent Accumulation of Iron by Isolated Rat Liver Mitochondria. IV. Relationship to the Energy State of the Mitochondria

Energy-dependent Accumulation of Iron by Isolated Rat Liver Mitochondria. IV. Relationship to the Energy State of the Mitochondria

I Romslo

Biochim Biophys Acta. 1975 Apr 14;387(1):69-79.

PMID: 1125288

Abstract:

1. The energy-dependent accumulation of iron by isolated rat liver mitochondria, respiring on endogenous substrates, is strongly dependent on the efficiency of energy coupling in the respiratory chain as measured by respiratory control with ADP and the endogenous energy dissipation. The accumulation reached a saturation level at respiratory control with ADP values (with succinate as the substrate) of approx. 4.0. 2. In the presence of exogenous substrate, the energy-dependent accumulation of iron was markedly reduced, primarily due to binding of iron as carboxylate complexes having less favourable constants than the iron (III)-sucrose complex(es). 3. The effect of added ATP was at least 2-fold, i.e. that of providing energy and that of chelating iron. When the mitochondria respired on endogenous substrate, the energy-dependent accumulation of iron increased at low concentrations of ATP, whereas higher concentrations (greater than 50 mu M) gradually inhibited the uptake. 4. Energization of the mitochondria by the generation of an artificial K-+ gradient across the inner membrane with valinomycin in a K-+-free medium increased the energy-dependent accumulation iron.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25440146-A	Iron(III) ionophore IV		25440-14-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: