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Energy Requirements for Protein Secretion via the Flagellar Type III Secretion System

Marc Erhardt

Methods Mol Biol. 2017;1615:449-457.

PMID: 28667628

Abstract:

Protein transport across the cytoplasmic membrane is coupled to energy derived from adenosine triphosphate hydrolysis or the protein motive force (pmf). A sophisticated, multi-component type III secretion system exports substrate proteins of both the bacterial flagellum and virulence-associated injectisome system of many Gram-negative pathogens. The type-III secretion system is primarily a pmf-driven protein exporter. Here, I describe methods to investigate the export of substrate proteins into the culture supernatant under conditions that manipulate the pmf.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP99348397 Potassium ionophore III Potassium ionophore III 99348-39-7 Price
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