Home
Resources
Publications
Engineering of Neuron Growth and Enhancing Cell-Chip Communication via Mixed SAMs

Engineering of Neuron Growth and Enhancing Cell-Chip Communication via Mixed SAMs

Aleksandr Markov, Vanessa Maybeck, Nikolaus Wolf, Dirk Mayer, Andreas Offenhäusser, Roger Wördenweber

ACS Appl Mater Interfaces. 2018 Jun 6;10(22):18507-18514.

PMID: 29763286

Abstract:

The interface between cells and inorganic surfaces represents one of the key elements for bioelectronics experiments and applications ranging from cell cultures and bioelectronics devices to medical implants. In the present paper, we describe a way to tailor the biocompatibility of substrates in terms of cell growth and to significantly improve cell-chip communication, and we also demonstrate the reusability of the substrates for cell experiments. All these improvements are achieved by coating the substrates or chips with a self-assembled monolayer (SAM) consisting of a mixture of organic molecules, (3-aminopropyl)-triethoxysilane and (3-glycidyloxypropyl)-trimethoxysilane. By varying the ratio of these molecules, we are able to tune the cell density and live/dead ratios of rat cortical neurons cultured directly on the mixed SAM as well as neurons cultured on protein-coated SAMs. Furthermore, the use of the SAM leads to a significant improvement in cell-chip communications. Action potential signals of up to 9.4 ± 0.6 mV (signal-to-noise ratio up to 47) are obtained for HL-1 cells on microelectrode arrays. Finally, we demonstrate that the SAMs facilitate a reusability of the samples for all cell experiments with little re-processing.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2602348	(3-Glycidyloxypropyl)triethoxysilane		2602-34-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: