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Enhanced Uptake of Plasmid at Boronic Acid Decorated Linear Polyethylenimines Results in Higher Transfection Efficiency

Enhanced Uptake of Plasmid at Boronic Acid Decorated Linear Polyethylenimines Results in Higher Transfection Efficiency

Santosh Yadav, Pradeep Kumar

Biointerphases. 2018 Nov 20;13(6):061003.

PMID: 30458622

Abstract:

High molecular weight polyethylenimines (PEIs) are considered as gold standard for transfection studies; however, cytotoxicity associated with branched ones and lower charge density on linear PEIs as well as lower uptake of the resulting deoxyribonucleic acid (DNA) complexes have limited their applications in clinical studies. In order to address these concerns and improve the uptake efficiency of the DNA complexes of linear polyethylenimine (25 kDa), the polymer was grafted with variable amounts of butylboronic acid to obtain a small series of linear polyethylenimine-butylboronic acid polymers. These modified polymers were allowed to interact with plasmid DNA and the resulting complexes were characterized by physicochemical techniques. Dynamic light scattering data showed the formation of nanosized complexes with positive zeta potential values. Furthermore, when these complexes were evaluated in vitro, they not only showed enhanced cell viability but also exhibited higher transfection efficiency as compared to native linear and branched PEIs and a commercially available standard transfection reagent, Lipofectamine 2000.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4426475	Butylboronic acid		4426-47-5	Price

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