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Enhancing Glutaric Acid Production in Escherichia Coli by Uptake of Malonic Acid

Enhancing Glutaric Acid Production in Escherichia Coli by Uptake of Malonic Acid

Xue Sui, Mei Zhao, Yingli Liu, Jing Wang, Guohui Li, Xiaojuan Zhang, Yu Deng

J Ind Microbiol Biotechnol. 2020 Mar;47(3):311-318.

PMID: 32140931

Abstract:

Glutaric acid is an important organic acid applied widely in different fields. Most previous researches have focused on the production of glutaric acid in various strains using the 5-aminovaleric acid (AMV) or pentenoic acid synthesis pathways. We previously utilized a five-step reversed adipic acid degradation pathway (RADP) in Escherichia coli BL21 (DE3) to construct strain Bgl146. Herein, we found that malonyl-CoA was strictly limited in this strain, and increasing its abundance could improve glutaric acid production. We, therefore, constructed a malonic acid uptake pathway in E. coli using matB (malonic acid synthetase) and matC (malonic acid carrier protein) from Clover rhizobia. The titer of glutaric acid was improved by 2.1-fold and 1.45-fold, respectively, reaching 0.56 g/L and 4.35 g/L in shake flask and batch fermentation following addition of malonic acid. Finally, the highest titer of glutaric acid was 6.3 g/L in fed-batch fermentation at optimized fermentation conditions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS7411350	4arm-PEG10K-Glutaric Acid			Price

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