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Enriching C-terminal Peptide From Endopeptidase ArgC Digest for Protein C-terminal Analysis

Enriching C-terminal Peptide From Endopeptidase ArgC Digest for Protein C-terminal Analysis

Hiroki Kuyama, Chihiro Nakajima, Koichi Tanaka

Bioorg Med Chem Lett. 2012 Dec 1;22(23):7163-8.

PMID: 23079528

Abstract:

This Letter describes a method for enriching C-terminal peptide of protein for C-terminal sequence analysis. This method employs endopeptidase ArgC digestion and C-terminal peptide enrichment using m-aminophenylboronic acid-agarose as an arginine-capture material. The selectively recovered C-terminal peptide incorporates no artificial derivatization. Therefore, the widely used functional groups (e.g. α-NH(2) and α-COOH) can be used for any necessary transformation. In this research, a TMPP mass tag was attached to the α-NH(2) group to clarify the amino acid sequence of the C-terminal peptide.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793853	m-Aminophenylboronic acid-Agarose			Price

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