Home
Resources
Publications
Enzymatic Cascades for Tailored 13 C 6 and 15 N Enriched Human Milk Oligosaccharides

Enzymatic Cascades for Tailored 13 C 6 and 15 N Enriched Human Milk Oligosaccharides

Thomas Fischöder, Samanta Cajic, Valerian Grote, Raphael Heinzler, Udo Reichl, Matthias Franzreb, Erdmann Rapp, Lothar Elling

Molecules. 2019 Sep 25;24(19):3482.

PMID: 31557948

Abstract:

Several health benefits, associated with human milk oligosaccharides (HMOS), have been revealed in the last decades. Further progress, however, requires not only the establishment of a simple "routine" method for absolute quantification of complex HMOS mixtures but also the development of novel synthesis strategies to improve access to tailored HMOS. Here, we introduce a combination of salvage-like nucleotide sugar-producing enzyme cascades with Leloir-glycosyltransferases in a sequential pattern for the convenient tailoring of stable isotope-labeled HMOS. We demonstrate the assembly of [13C6]galactose into lacto-N- and lacto-N-neo-type HMOS structures up to octaoses. Further, we present the enzymatic production of UDP-[15N]GlcNAc and its application for the enzymatic synthesis of [13C6/15N]lacto-N-neo-tetraose for the first time. An exemplary application was selected-analysis of tetraose in complex biological mixtures-to show the potential of tailored stable isotope reference standards for the mass spectrometry-based quantification, using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) as a fast and straightforward method for absolute quantification of HMOS. Together with the newly available well-defined tailored isotopic HMOS, this can make a crucial contribution to prospective research aiming for a more profound understanding of HMOS structure-function relations.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13007324	Lacto-N-neo-tetraose		13007-32-4	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: