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Enzymatic Production of low-Mw Chitosan-Derivatives: Characterization and Biological Activities Evaluation

Enzymatic Production of low-Mw Chitosan-Derivatives: Characterization and Biological Activities Evaluation

Sawsan Affes, Hana Maalej, Inmaculada Aranaz, Niuris Acosta, Ángeles Heras, Moncef Nasri

Int J Biol Macromol. 2020 Feb 1;144:279-288.

PMID: 31830447

Abstract:

This study reports the enzymatic depolymerization of chitosan by the chitosanolytic preparation of Bacillus licheniformis GA11. For this purpose, chitosanase production and biochemical characterization of the crude enzyme preparation from GA11 were firstly investigated. The highest chitosanase production was obtained in a culture medium containing 50.0 g/l of a mixture of soluble starch/shrimp shells/crab shells and 5.0 g/l tryptone, after incubation during 5 days, at 30 °C with a pHi of 6.0 and under continuous agitation at 200 rpm. Then, the chitosanolytic preparation, exhibiting maximum activity at 65 °C and pH 5.0, was used to hydrolyze chitosan, leading to various chitosan-depolymerization products (CDP) with different physicochemical characteristics. Finally, the antioxidant and antimicrobial potentials of CDP were evaluated, allowing to conclude that the molecular weight and the acetylation degree highly affect the biological activities of CDP.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP851053640	GA11		851053-64-0	Price

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