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Enzymatic Synthesis of Isotopically Labeled Isoprenoid Diphosphates

D J Christensen, C D Poulter

Bioorg Med Chem. 1994 Jul;2(7):631-7.

PMID: 7858969

Abstract:

Recombinant yeast isopentenyl diphosphate (IPP) isomerase and avian farnesyl diphosphate (FPP) synthase from overproducing strains of Escherichia coli were used to synthesize FPP from IPP and dimethylallyl diphosphate (DMAPP). [2,4,5-13C3]IPP and [2,4,5-13C3]DMAPP were synthesized from ethyl [2-13C]bromoacetate and [1,3-13C2]acetone. Thes compounds were used as substrates for enzymatic synthesis of FPP selectivity labeled at the first or third isoprene residue or at all three.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42410459 Trimethylolpropane-3,4,5-13C3 phosphate Trimethylolpropane-3,4,5-13C3 phosphate Price
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