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Enzyme Activity in Dialkyl Phosphate Ionic Liquids

Marie F Thomas, Luen-Luen Li, Jocelyn M Handley-Pendleton, Daniel van der Lelie, John J Dunn, James F Wishart

Bioresour Technol. 2011 Dec;102(24):11200-3.

PMID: 22001053

Abstract:

The activity of four metagenomic enzymes and an enzyme cloned from the straw mushroom, Volvariella volvacea were studied in the following ionic liquids, 1,3-dimethylimidazolium dimethyl phosphate, [mmim][dmp], 1-ethyl-3-methylimidazolium dimethyl phosphate, [emim][dmp], 1-ethyl-3-methylimidazolium diethyl phosphate, [emim][dep] and 1-ethyl-3-methylimidazolium acetate, [emim][OAc]. Activity was determined by analyzing the hydrolysis of para-nitrobenzene carbohydrate derivatives. In general, the enzymes were most active in the dimethyl phosphate ionic liquids, followed by acetate. Generally speaking, activity decreased sharply for concentrations of [emim][dep] above 10% v/v, while the other ionic liquids showed less impact on activity up to 20% v/v.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP848641690 1-Ethyl-3-methylimidazolium diethyl phosphate 1-Ethyl-3-methylimidazolium diethyl phosphate 848641-69-0 Price
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