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Enzyme-linked Immunosorbent Assay for the Detection of Total Cathepsin H in Human Tissue Cytosols and Sera

Enzyme-linked Immunosorbent Assay for the Detection of Total Cathepsin H in Human Tissue Cytosols and Sera

A Schweiger, B Stabuc, T Popovíc, V Turk, J Kos

J Immunol Methods. 1997 Feb 28;201(2):165-72.

PMID: 9050938

Abstract:

An enzyme-linked immunosorbent assay (ELISA) was constructed for the determination of total human cathepsin H concentration in clinical samples. Utilising monoclonal and polyclonal antibodies, raised to human liver cathepsin H, the assay is able to detect a mature protein, a precursor molecule and enzyme-inhibitor complexes. The test system permits sensitive and reliable detection of analyte either in tissue cytosols or in sera. The detection limit is 2 ng/ml (n = 10, mean of zero standard +/- 3 SD). The average recovery of cathepsin H, added to the low content samples, was 95.3% +/- 1.8%. The within-run and between-run coefficient of variance (CV) varied from 2.3% to 8.9% and 12.7% to 16.4%, respectively, indicating satisfactory reproducibility of the method. The level of cathepsin H was defined in tissue cytosols of human heart, muscle and kidney and in sera from 30 healthy individuals. Additionally, cathepsin H was measured in sera from 55 patients with primary skin melanoma and from 42 patients with metastatic melanoma. The mean cathepsin H level was significantly higher for both groups of patients compared to normal sera level, being highest for metastatic melanoma patients.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP60748734	Cathepsin H, Human Liver		60748-73-4	Price

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