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Enzyme-resistant Isomalto-Oligosaccharides Produced From Leuconostoc Mesenteroides NRRL B-1426 Dextran Hydrolysis for Functional Food Application

Enzyme-resistant Isomalto-Oligosaccharides Produced From Leuconostoc Mesenteroides NRRL B-1426 Dextran Hydrolysis for Functional Food Application

Damini Kothari, Arun Goyal

Biotechnol Appl Biochem. 2016 Jul;63(4):581-9.

PMID: 25939683

Abstract:

The extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1426 was produced and purified using polyethylene glycol fractionation. In our earlier study, it was reported that L. mesenteroides dextransucrase synthesizes a high-molecular mass dextran (>2 × 10(6) Da) with ∼85.5% α-(1→6) linear and ∼14.5% α-(1→3) branched linkages. Isomalto-oligosaccharides (IMOs) were synthesized through depolymerization of dextran by the action of dextranase. The degree of polymerization of IMOs was 2-10 as confirmed by mass spectrometry. The nuclear magnetic resonance spectroscopic analysis revealed the presence of α-(1→3) linkages in the synthesized IMOs. The IMOs were resistant to dextranase, α-glucosidase, and α-amylase, and therefore can have potential application as food additives in the functional foods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9004540-A	Dextran from Leuconostoc mesenteroides		9004-54-0	Price

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