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Epidermal Growth Factor Stimulates 3-hydroxy-3-methylglutaryl-coenzyme A Reductase Expression via the ErbB-2 Pathway in Human Breast Adenocarcinoma Cells

Epidermal Growth Factor Stimulates 3-hydroxy-3-methylglutaryl-coenzyme A Reductase Expression via the ErbB-2 Pathway in Human Breast Adenocarcinoma Cells

R Asslan, A Pradines, C Pratx, C Allal, G Favre, F Le Gaillard

Biochem Biophys Res Commun. 1999 Jul 14;260(3):699-706.

PMID: 10403829

Abstract:

HMG-CoA reductase is the key enzyme for the biosynthesis of isoprenoid compounds essential for cell growth and differentiation. Its tyrosine kinase-dependent modulation has recently been suggested and described in the ErbB-2 overexpressing cell line SKBR-3 [Asslan et al. (1998) Biochem. J. 330, 241-246]. Epidermal growth factor (EGF) increased the HMG-CoA reductase activity, protein, and mRNA levels only in ErbB-2-expressing cells (SKBR-3 and MCF-7) but not in MDA-MB-468 cells that do not express ErbB-2 even though their EGF receptor was efficiently phosphorylated. Tyrphostin AG 879, a specific inhibitor of ErbB-2 tyrosine kinase activity, decreased HMG-CoA reductase activity only in cells that expressed ErbB-2. A functional EGF receptor appeared to be necessary since its inhibition by the specific tyrphostin AG 1478 abolished the EGF effects. Phosphatidylinositol 3-kinase (PI 3-kinase) might be a crucial enzyme in the signaling pathway since the specific inhibitor, LY 294002, was shown to inhibit HMG-CoA reductase activity and to completely abolish the stimulation by EGF in SKBR-3 cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP148741304-B	Tyrphostin AG 879		148741-30-4	Price

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