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Equivalency of Endothelial Cell Growth Supplement to Irradiated Feeder Cells in Carcinogen-Induced Morphologic Transformation of Syrian Hamster Embryo Cells

Equivalency of Endothelial Cell Growth Supplement to Irradiated Feeder Cells in Carcinogen-Induced Morphologic Transformation of Syrian Hamster Embryo Cells

C H Evans, J A DiPaolo

J Natl Cancer Inst. 1982 Jan;68(1):127-31.

PMID: 6275157

Abstract:

Endothelial cell growth supplement (ECGS), an extract of bovine neural tissue with growth-promoting properties for human endothelial and epithelial cells and for mouse BALB/c fibroblast-like cells, can be substituted for feeder cells in a quantitative 7-day Syrian hamster embryo cell colony in vitro model of carcinogenesis. Inclusion of 50 or 100 micrograms ECGS/ml medium throughout the 7-day growth period produced results equal to those obtained with feeder cells. The frequency and morphology of normal fibroblast colonies and carcinogen-induced morphologically transformed cell colony growth in the presence of ECGS were similar to those in the presence of feeder cells. A positive dose-response relationship in transformation by benzo[a]pyrene occurred. The frequency of transformed colonies following UV irradiation and treatment of the cells with the cocarcinogenic tumor promoter 12-O-tetradecanoylphorbol 13-acetate was greatly augmented, and lymphotoxin, a lymphokine with anticarcinogenic activity, reduced transformation. Thus ECGS can substitute for feeder cells in supporting in vitro transformation and eliminates a potential complex source of variability for studies where interaction(s) with feeder cells are a consideration. The mechanics of this model system was simplified, and its versatility for the study of physiologic, carcinogenic, and other pathophysiologic processes was broadened.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248871	Endothelial cell growth supplement from bovine neural tissue			Price

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