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Establishment and Characterization of a Parietal Endoderm-Like Cell Line Derived From Engelbreth-Holm-Swarm Tumor (EHSPEL), a Possible Resource for an Engineered Basement Membrane Matrix

Establishment and Characterization of a Parietal Endoderm-Like Cell Line Derived From Engelbreth-Holm-Swarm Tumor (EHSPEL), a Possible Resource for an Engineered Basement Membrane Matrix

Yoshitaka Hayashi, Tomomi Emoto, Sugiko Futaki, Kiyotoshi Sekiguchi

Matrix Biol. 2004 Apr;23(1):47-62.

PMID: 15172037

Abstract:

Engelbreth-Holm-Swarm (EHS) tumor produces large amounts of basement membrane (BM) components, which are widely used as cell culture substrates mimicking BM functions. EHS tumor arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation. In the present study, we established a cell line, EHSPEL (EHS Parietal Endoderm-Like), which can be cultured ex vivo and preserves the capacity to form tumors in vivo. EHSPEL cells secreted large amounts of laminin-1 into the medium and deposited BM components onto dishes. To further characterize EHSPEL cells, their gene expression profile was compared to those of parietal endoderm cells from Reichert's membrane at embryonic day 13.5, differentiated F9 embryonal carcinoma cells, and PYS-2 parietal endoderm cells. These analyses outlined not only common features of parietal endoderm-like cells that underlie the efficient production of BM components, but also germline cell-like features of EHSPEL cells, at least some of which may play crucial roles in their capacity to form tumors that accumulate abundant BM components in vivo. Karyotyping of EHSPEL cells using chromosome painting probes showed a large number of interchromosomal rearrangements and partial chromosome hyperploidy. Exogenous introduction of a human laminin-alpha(4)-EGFP fusion protein into EHSPEL cells resulted in the production and deposition of human-mouse-hybrid laminin-8. This strategy should be applicable for creating efficient systems to produce chimeric laminins as well as BM-like gels with modified biological activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP114956819-A	Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane		114956-81-9	Price

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