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Establishment and Characterization of Calcyclin Binding Protein (CacyBP) Monoclonal Antibody

Establishment and Characterization of Calcyclin Binding Protein (CacyBP) Monoclonal Antibody

Huihong Zhai, Yongquan Shi, Jinhua Yu, Liu Hong, Hongwei Tang, Jun Wang, Shengjuan Hu, Feihu Bai, Daiming Fan

Hybridoma (Larchmt). 2006 Apr;25(2):91-4.

PMID: 16704310

Abstract:

Anti-calcyclin binding protein (CacyBP) monoclonal antibodies (MAb) were produced using an in vitro immunization method. BALB/c mouse splenocytes were immunized with purified 6 x His-CacyBP fusion protein and fused with myeloma cells using polyethylene glycol (PEG) 4000. By selection using enzyme-linked immunosorbent assay (ELISA), three anti-CacyBP MAbs were obtained. The MAb BD1, whose isotype was IgG1, interacted with the fusion protein. Western blot and immunofluorescence microscopy showed that the MAb BD1 against CacyBP could recognize CacyBP protein derived from human gastric cancer cell lines in both native and denatured forms. This MAb would act as a useful tool for the detection of CacyBP protein in future studies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415037	Calcyclin Binding Protein human			Price
IAR4249266	Monoclonal Anti-CACYBP antibody produced in mouse			Price

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