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Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic Acid mono-N-hydroxysuccinimide Ester (DOTA-NHS-ester) Based Lectin Microarray for Efficiently Detecting Serum Glycans in Gastric Cancers

Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic Acid mono-N-hydroxysuccinimide Ester (DOTA-NHS-ester) Based Lectin Microarray for Efficiently Detecting Serum Glycans in Gastric Cancers

Yi Gao, Song-Guo Li, Qian Liu, Sheng-Sheng Liu, Lei Ye, Zi-Jian Song, Wei-Dong Du

Anal Biochem. 2020 May 15;597:113686.

PMID: 32156505

Abstract:

Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA-NHS-ester is brought about via activating three -OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P < 0.05). The biochip would provide a versatile platform for investigation of potential glycan biomarkers in making tumor diagnosis decision and analyzing escape of tumors from immunity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1263190700	16-Amino-1-hexadecanethiol hydrochloride		1263190-70-0	Price

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