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Establishment of a Phagocytic Cell Line From Bombina Orientalis

Establishment of a Phagocytic Cell Line From Bombina Orientalis

S C Park, S H Lee, S S Han

Methods Cell Sci. 2000 Mar;22(1):1-7.

PMID: 10650327

Abstract:

Continuous serum-free culture of Bok-2 cells was generated from primary culture of the tail bud stage embryos of the toad, Bombina orientalis. Bok-2 cells can be maintained in modified L-15 serum-free medium prepared by mixing L-15 medium, lactalbumin enzymatic hydrolysate, sucrose and sodium bicarbonate. Bok-2 cells have an ameboid behavior and morphology. When Bok-2 and viable Candida albicans were co-cultured, Bok-2 cells showed an immune response characterized by chemotaxis, phagocytosis and partial clearing activity of Candida cells and colonies. And Bok-2 cells also showed phagocytosis of latex beads without serum treatment and displayed numerous pseudopodia, membrane evagination for phagocytosis and phagosome activity. On the basis of these results, Bok-2 cells were identified as professional phagocytes.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP68458877	Lactalbumin enzymatic hydrolysate		68458-87-7	Price

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