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Estimation of Lecithin in Rabbit Tissues

J E Gardiner, H S Lee

Clin Exp Pharmacol Physiol. Jan-Feb 1982;9(1):53-6.

PMID: 7094433

Abstract:

1. A simple method for lecithin estimation was developed. Phospholipids in rabbit tissues were extracted with chloroform and methanol. 2. The samples were chromatographed with eluting solvent system; chloroform:methanol:glacial acetic acid:water (100:25:8:1.1). Lecithin was identified by the spray reagent as blue spots and the intensities were quantitated with a densitometer. 3. The concentration of lecithin in the various rabbit tissues varied from 2.8 to 10 mg/g tissue. The sequence of increasing lecithin concentration was heart less than kidney less than lung less than liver less than brain. 4. Recovery experiments of added lecithin into the tissues showed a recovery of 91-104%.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
DR5605 Molybdenum Blue spray reagent Molybdenum Blue spray reagent Price
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