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Ethyl Linoleate From Garlic Attenuates Lipopolysaccharide-Induced Pro-Inflammatory Cytokine Production by Inducing Heme oxygenase-1 in RAW264.7 Cells

Ethyl Linoleate From Garlic Attenuates Lipopolysaccharide-Induced Pro-Inflammatory Cytokine Production by Inducing Heme oxygenase-1 in RAW264.7 Cells

Sun Young Park, Rajasekar Seetharaman, Min Jung Ko, Do Yeon Kim, Tae Hoon Kim, Moo Kyoung Yoon, Jung Ho Kwak, Sang Joon Lee, Yoe Sik Bae, Young Whan Choi

Int Immunopharmacol. 2014 Apr;19(2):253-61.

PMID: 24508058

Abstract:

In the present study, an essential fatty acid, ethyl linoleate (ELA), was isolated from the cloves of Allium sativum, and its structure was elucidated by NMR and GC-MS analyses. In vitro systems were used to evaluate the anti-inflammatory activity of ELA. Our results indicate that ELA down-regulates inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression and thereby reduces nitric oxide (NO) and prostaglandin E2 production in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Immunofluorescent microscopy and western blot analyses revealed that these effects were mediated by impaired translocation of nuclear factor (NF)-κB and inhibition of phosphorylation of mitogen activated protein kinases. Furthermore, ELA exerted its anti-inflammatory activity by inducing heme oxygenase-1 (HO-1) expression, as determined by HO-1 small interfering (Si) RNA system. Si RNA-mediated knock-down of HO-1 abrogated the inhibitory effects of ELA on the production of NO, TNF-α, IL-1β, and IL-6 in LPS-induced macrophages. These findings indicate the potential therapeutic use of ELA as an anti-inflammatory agent.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP544354-A	Ethyl linoleate		544-35-4	Price

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