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Evaluating the Effects on Steroidogenesis of Estragole and Trans-Anethole in a Feto-Placental Co-Culture Model

Evaluating the Effects on Steroidogenesis of Estragole and Trans-Anethole in a Feto-Placental Co-Culture Model

Debbie Yancu, Cathy Vaillancourt, J Thomas Sanderson

Mol Cell Endocrinol. 2019 Dec 1;498:110583.

PMID: 31536780

Abstract:

In this study, we determined whether estragole and its isomer trans-anethole interfered with feto-placental steroidogenesis in a human co-culture model composed of fetal-like adrenocortical (H295R) and placental trophoblast-like (BeWo) cells. Estragole and trans-anethole are considered the biologically active compounds within basil and fennel seed essential oils, respectively. After a 24 h exposure of the co-culture to 2.5, 5.2 and 25 μM estragole or trans-anethole, hormone concentrations of estradiol, estrone, dehydroepiandrosterone, androstenedione, progesterone and estriol were significantly increased. Using RT-qPCR, estragole and trans-anethole were shown to significantly alter the expression of several key steroidogenic enzymes, such as those involved in cholesterol transport and steroid hormone biosynthesis, including StAR, CYP11A1, HSD3B1/2, SULT2A1, and HSD17B1, -4, and -5. Furthermore, we provided mechanistic insight into the ability of estragole and trans-anethole to stimulate promoter-specific expression of CYP19 through activation of the PKA pathway in H295R cells and the PKC pathway in BeWo cells, in both cases associated with increased cAMP levels. Moreover, we show new evidence suggesting a role for progesterone in regulating steroid hormone biosynthesis through regulation of the StAR gene.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP140670-A	Estragole		140-67-0	Price
AP4180238	Anethole		4180-23-8	Price

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