0

Evaluation of 2,6-dihydroxyacetophenone as Matrix-Assisted Laser desorption/ionization Matrix for Analysis of Hydrophobic Proteins and Peptides

Johan Jacksén, Asa Emmer

Anal Biochem. 2012 Jun 1;425(1):18-20.

PMID: 22406432

Abstract:

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for analysis of macromolecules like peptides and proteins. The analysis procedure is generally simple but must be adapted to the characteristics of the analytes. Therefore, specific matrices suitable for, e.g., hydrophobic proteins and peptides that are difficult to analyze would be preferable in order to optimize the outcome. In the present work, 2,6-dihydroxyacetophenone (DHAP) was shown to be beneficial in comparison to DHB for intact bacteriorhodopsin (BR) as well as for chemically digested BR.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP699832 2′,6′-Dihydroxyacetophenone 2′,6′-Dihydroxyacetophenone 699-83-2 Price
qrcode