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Evaluation of Matrix Effects on TOF-SIMS Data of Leu-Enkephalin and 1,2-dioleoyl-sn-glycero-3-phosphocholine Mixed Samples

Evaluation of Matrix Effects on TOF-SIMS Data of Leu-Enkephalin and 1,2-dioleoyl-sn-glycero-3-phosphocholine Mixed Samples

Shusuke Nakano, Takayuki Yamagishi, Satoka Aoyagi, André Portz, Michael Dürr, Hideo Iwai, Tomoko Kawashima

Biointerphases. 2018 Jan 19;13(3):03B403.

PMID: 29351722

Abstract:

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is one of the most powerful methods to analyze biomolecules in biological tissues and cells because it provides detailed chemical structure information and chemical images with a high spatial resolution. However, in terms of quantitative analysis, there are issues such as matrix effects that often cause secondary ion intensity changes regardless of the actual concentration in a sample. For instance, the intensity of secondary ions related to peptides is generally suppressed when lipids coexist. Since the evaluation of biomolecules is crucial to understand biological phenomena, it is required to analyze peptides or lipids without matrix effects. Therefore, the mechanism of matrix effects regarding peptides and lipids in TOF-SIMS was investigated in this study. Leu-enkephalin (YGGFL, molecular weight of 555.3 Da) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, C44H84NO8P, molecular weight 785.6 Da) were employed to prepare model samples. Model samples contain different weight ratios of these two molecules. The intensity of secondary ions related to the peptide or the lipid was compared with control samples containing pure leu-enkephalin or DOPC. As a result, it is indicated that the intensity of DOPC related secondary ions is strongly enhanced by coexisting leu-enkephalin, while the intensity of leu-enkephalin related secondary ions is suppressed by coexisting DOPC especially in a low concentration range of the peptide.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP4235954	1,2-Dioleoyl-sn-glycero-3-phosphocholine		4235-95-4	Price

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