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Evaluation of OMNIgene

Evaluation of OMNIgene

J Zallet, I D Olaru, A-K Witt, P Vock, B Kalsdorf, S Andres, D Hillemann, K Kranzer

Int J Tuberc Lung Dis. 2018 Aug 1;22(8):945-949.

PMID: 29991406

Abstract:

Setting:
National Mycobacterium Reference Laboratory, Borstel, Germany.
Objective:
To evaluate the effectiveness of OMNIgene®•SPUTUM (OM-S) reagent in comparison with a method using N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) with regard to mycobacterial recovery and contamination of broth and solid cultures.
Design:
Sputum samples from patients with tuberculosis and other respiratory diseases underwent decontamination with NALC-NaOH-based (MycoDDR™) or OM-S reagent. The decontamination procedure was assigned by block randomisation. Samples were inoculated on Löwenstein-Jensen, Stonebrink and MGIT™ (Mycobacterial Growth Indicator Tubes). Mycobacterial recovery from samples spiked with Mycobacterium tuberculosis following decontamination was determined.
Results:
Eighty-five samples were randomised to NALC-NaOH and 84 to OM-S reagent. Mycobacterial recovery was significantly lower for samples processed with OM-S reagent compared with the NALC-NaOH method across all media types. Culture contamination was lower with NALC-NaOH reagent on solid media (9.4-12.9% vs. 28.6-29.8%). Growth was not observed in MGIT among samples spiked with 10 600-16 800 colony-forming units of M. tuberculosis following decontamination with OM-S reagent.
Conclusion:
Low mycobacterial recovery, especially in MGIT, observed in the present study suggests that OM-S reagent might not be compatible with the MGIT system. More extensive field evaluations of the OM-S reagent are warranted to demonstrate a significant benefit over currently used methods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP8007098	Eschka's Reagent		8007-09-8	Price

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