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Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis

Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis

Yanhong Bi, Zhangqun Duan, Wenjing Zhang, Lei Xu, Zhaoyu Wang, Xiaojuan Zhao, Xiangjie Zhao, Jiali Yang

Biomed Res Int. 2019 May 20;2019:4318631.

PMID: 31236406

Abstract:

In this study, comparative experiments were carried out to investigate the positional selectivity of Candida sp. 99-125 lipase in preparing 1,3-diolein by using medium engineering strategy. The results indicated that the diolein yield was markedly enhanced from 56.5% to 86.7% with increasing log⁡P values of the solvents, while the selectivity of the examined lipase for the sn-1 over the sn-2 hydroxyl of glycerol was decreased, thus leading to a reduced 1,3-diolein to 1,2-diolein ratio. To evaluate the possibility of industrial enzymatic production of 1,3-diolein, larger-scale experiments were assessed. After being used repeatedly for eight batches, the diolein content reached 95.1%, while the 1,3-diolein to 1,2-diolein ratio was 7:1 following purification. Results of the kg level experiments significantly demonstrated the practicability of the enzymatic process and the efficiency of the purification strategy for the product.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2465329	1,3-Diolein		2465-32-9	Price
IAR424808	Lipase from Candida sp.			Price

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