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Evaluation of the Effect of Butyl P-Hydroxybenzoate on the Proteolytic Activity and Membrane Function of Human Spermatozoa

Evaluation of the Effect of Butyl P-Hydroxybenzoate on the Proteolytic Activity and Membrane Function of Human Spermatozoa

B L Song, D R Peng, H Y Li, G H Zhang, J Zhang, K L Li, Y Q Zhao

J Reprod Fertil. 1991 Mar;91(2):435-40.

PMID: 2013872

Abstract:

The inhibition of the proteolytic activity of acrosin in human spermatozoa by butyl p-hydroxybenzoate was assessed by the gelatin substrate film method. Compared with a typical acrosin inhibitor, TLCK, the inhibitory activity of butyl p-hydroxybenzoate to acrosin was much more effective (20 times) than that of TLCK, proving that butyl p-hydroxybenzoate was a potent acrosin inhibitor. The effect of butyl p-hydroxybenzoate on membrane function of human spermatozoa was evaluated using a sperm-tail hypoosmotic swelling test and supravital stain method. A good correlation (r = 0.92) was observed between the % spermatozoa with normal membrane function and the % live spermatozoa after treatment of the spermatozoa with butyl p-hydroxybenzoate for 1 min, indicating that the death of spermatozoa caused by butyl p-hydroxybenzoate is probably due to impairment of sperm membrane function. Both the inhibitory effect on acrosin and the adverse effect on membrane function suggest that butyl p-hydroxybenzoate could be developed as a new vaginal contraceptive.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411004	Butyl p-aminobenzoate-Agarose			Price

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