Home
Resources
Publications
Evolutionary Classification of CRISPR-Cas Systems: A Burst of Class 2 and Derived Variants

Evolutionary Classification of CRISPR-Cas Systems: A Burst of Class 2 and Derived Variants

Kira S Makarova, Yuri I Wolf, Jaime Iranzo, Sergey A Shmakov, Omer S Alkhnbashi, Stan J J Brouns, Emmanuelle Charpentier, David Cheng, Daniel H Haft, Philippe Horvath, Sylvain Moineau, Francisco J M Mojica, etc.

Nat Rev Microbiol. 2020 Feb;18(2):67-83.

PMID: 31857715

Abstract:

The number and diversity of known CRISPR-Cas systems have substantially increased in recent years. Here, we provide an updated evolutionary classification of CRISPR-Cas systems and cas genes, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015. The new classification includes 2 classes, 6 types and 33 subtypes, compared with 5 types and 16 subtypes in 2015. A key development is the ongoing discovery of multiple, novel class 2 CRISPR-Cas systems, which now include 3 types and 17 subtypes. A second major novelty is the discovery of numerous derived CRISPR-Cas variants, often associated with mobile genetic elements that lack the nucleases required for interference. Some of these variants are involved in RNA-guided transposition, whereas others are predicted to perform functions distinct from adaptive immunity that remain to be characterized experimentally. The third highlight is the discovery of numerous families of ancillary CRISPR-linked genes, often implicated in signal transduction. Together, these findings substantially clarify the functional diversity and evolutionary history of CRISPR-Cas.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP954143487	Meriolin 3 - CAS 954143-48-7		954143-48-7	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: