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Exendin-4 Plays a Protective Role in a Rat Model of Spinal Cord Injury Through SERCA2

Exendin-4 Plays a Protective Role in a Rat Model of Spinal Cord Injury Through SERCA2

Zhonglei Sun, Yingfu Liu, Xianbin Kong, Renjie Wang, Yunqiang Xu, Chongzhi Shang, Jingrui Huo, Mengqiang Huang, Fei Zhao, Kefeng Bian, Sai Zhang, Yue Tu, Xuyi Chen

Cell Physiol Biochem. 2018;47(2):617-629.

PMID: 29794460

Abstract:

Background/aims:
Current therapies for spinal cord injury (SCI) have limited efficacy, and identifying a therapeutic target is a pressing need. Sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 2 (SERCA2) plays an important role in regulating calcium homeostasis, which has been shown to inhibit apoptosis. Exendin-4 has been shown to inhibit the apoptosis of nerve cells in SCI, which can also improve SERCA2 expression. In this study, we sought to determine whether exendin-4 plays a protective role in a rat model of SCI via SERCA2.
Methods:
To investigate the effects of exendin-4 on SCI, a rat model of SCI was induced by a modified version of Allen's method. Spinal cord tissue sections from rats and western blot analysis were used to examine SERCA2 expression after treatment with the long-acting glucagon-like peptide 1 receptor exendin-4 or the SERCA2 antagonist 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CE). Locomotor function was evaluated using the Basso Beattie Bresnahan locomotor rating scale and slanting board test.
Results:
Cell apoptosis was increased with CE treatment and decreased with exendin-4 treatment. Upregulation of SERCA2 in female rats with SCI resulted in an improvement of motor function scores and histological changes.
Conclusion:
These findings suggest that exendin-4 plays a protective role in a rat model of SCI through SERCA2 via inhibition of apoptosis. Existing drugs targeting SERCA2 may be an effective therapeutic strategy for the treatment of SCI.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP150347594	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester		150347-59-4	Price

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