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Exploration of Detergent-Stable Alkaline α-amylase AA7 From Bacillus Sp Strain SP-CH7 Isolated From Chilika Lake

Exploration of Detergent-Stable Alkaline α-amylase AA7 From Bacillus Sp Strain SP-CH7 Isolated From Chilika Lake

Sonali Priyadarshini, Pratima Ray

Int J Biol Macromol. 2019 Nov 1;140:825-832.

PMID: 31401271

Abstract:

An alkali-tolerant bacterial isolate CH7 was isolated from Chilika Lake sediments that produced α-amylase at high pH. It showed >15 mm zone diameter at pH 11.0 on agar plate containing 1% starch hence considered for study. It survived up to 10% NaCl concentration on nutrient agar plates. Taxonomically it was identified as Bacillus sp. strain SP-CH7 having GenBank Accession No. KU761266. Optimized alkaline α-amylase, AA7 showed 202.857 specific activity (IU/mg), 65.95 purification fold and 25.94 yield (%) respectively. Molecular weight was revealed by SDS-PAGE as 55 kDa. The Km value was 0.688 mg/mL and so was 2.342 IU/mL Vmax obtained for AA7 towards starch. This enzyme showed 92.79% and 91.14% stability at pH 11.0 and 13.0 respectively and up to 75 °C stability was also noted. Enzyme activity was stimulated by Co2+, Na+ and Ca2+. It was stable in presence of EDTA, SDS, all the powdered detergents as well as with liquid detergent "Ezee" which vouches for its potential application in Detergent industries.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9000855-D	α-Amylase from Bacillus sp.		9000-85-5	Price

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